Kev cai Peptide Hloov Kho|Peptide Tshuaj Hloov Kho| Fluorescent -labeled Peptide, Phosphorylated Peptide, Stapled Peptide|Kev tshawb fawb-Peptide
Meta Description
Xav tau peptides nrog kev hloov kho tshwj xeeb?Science-PeptideCustom Peptide Modification kev pabcuam npog ntau dua 200 hom kev hloov kho, xws li fluorescently sau npe, phosphorylated, glycosylated, PEGylated, stapled peptides, thiab lwm yam. Nrog 20 xyoo ntawm kev paub, peb muaj peev xwm muab kev hloov kho kom ruaj khov. Thov koj xav tiv tauj peb.
Xav tau peptide nrog kev hloov kho rau koj qhov kev tshawb fawb? Cia peb ua.
Vim li cas koj thiaj xav tau Kev Hloov Kho Peptide?
Thaum ua kev tshawb fawb, peb feem ntau yuav tsum tau ntxiv qee qhov "kev ua haujlwm tshwj xeeb" rau peptides - xws li txuas ib pab pawg fluorescent kom pom tias nws khiav hauv lub cell li cas, los yog ntxiv ib pab pawg phosphoryl los ua kom muaj zog ntawm cov proteins, lossis ua cov peptide stapled kom cov qauv ruaj khov thiab tsis yooj yim degraded los ntawm enzymes. degraded los ntawm enzymes.
Cov kev hloov kho no tsis nyuaj, tab sis thaum nws los txog rau synthesizing lawv, cov lus nug tshwm sim: cov chaw hloov kho puas yog? Puas yog cov qoob loo txaus? Puas yog purity zoo txaus? Puas yuav muaj ntau yam kev hloov kho ua ke sib ntaus sib tua?
Kev tshawb fawb-Peptide's Custom Peptide Modification service yog tshwj xeeb hauv kev daws cov teeb meem no, nrog 20 xyoo ntawm kev paub txog kev hloov pauv peptide thiab ntau tshaj 200 hom kev hloov kho, peb tuaj yeem nrhiav cov txheej txheem hluavtaws txhim khu kev qha rau cov ntawv sau biotin yooj yim lossis ntau yam kev hloov kho sib xyaw ua ke.
Hauv qab no yog qee qhov kev hloov kho uas peb txhawb nqa (ib feem ntawm cov npe):
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Hom Kev Hloov Kho |
Kev Hloov Kho |
Daim ntawv thov Scenario |
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Fluorescent Labeling |
FITC (Fluorescein Isothiocyanate), FAM (Fluorescein Amidite), TAMRA (Tetramethylrhodamine), Cy3 / Cy5, Rhodamine, Alexa Fluor series |
Cell duab, ntws cytometry, fluorescence polarization |
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Affinity Tagging |
Biotin, Nws-tag (Histidine tag), Chij-tag, HA-tag (Hemagglutinin tag) |
Affinity purification, rub-down assays, thiab SPR (Surface Plasmon Resonance) tsom xam |
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Kev hloov kho ruaj khov |
PEGylation (kev hloov kho nrog Polyethylene Glycol), hloov pauv cov roj fatty acid (piv txwv li, palmitic acid, myristic acid), hloov cov roj cholesterol |
Extension ntawm ib nrab -lub neej, txhim kho solubility, txhim kho daim nyias nyias permeability |
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Ncej -Kev Hloov Hloov Kev Hloov Hloov Mimicry (PTM mimicry) |
Phosphorylation (pSer: phosphoserine / pThr: phosphothreonine / pTyr: phosphotyrosine), glycosylation, acetylation, methylation |
Kev tshawb fawb qhia txoj hauv kev, kev txheeb xyuas cov enzyme substrate, protein-protein cuam tshuam |
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Kev hloov kho cov qauv |
Amidation, cyclization, stapled peptide (lub stabilized peptide los ntawm covalent txuas) |
Stabilized conformation, nce tsis kam mus rau proteolysis, txhim khu kev ua si |
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Kev koom ua ke ntawm Unnatural Amino Acids |
D-amino acids, non-natural amino acids, isotopic labeling (¹⁵N: nitrogen-15 / ¹³C: carbon-13) |
Structure–activity relationship (SAR) cov kev tshawb fawb, NMR (Nuclear Magnetic Resonance) kev tsom xam, kev ntsuam xyuas metabolic stability |
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Photosensitive sojntsuam |
Yees duab- pawg sib txuas (Bpa: p-Benzoyl-L-phenylalanine), pawg tiv thaiv photolabile (Nvoc: Nitroveratryloxycarbonyl) |
Photoaffinity labeling, yees duab-tswj tso tawm, spatiotemporal kev tshawb fawb tshwj xeeb |
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Precursors rau Radiolabeling |
DOTA (1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid), NOTA (1,4,7-Triazacyclononane-1,4,7-triacetic acid) rau hlau chelation |
PET / SPECT imaging (Positron Emission Tomography / Tib Photon Emission Computed Tomography), radiotherapeutic tshuaj txhim kho |
Vim li cas thiaj xaiv peb rau Kev Cai Peptide Hloov Kho?
1. Ntau tshaj 200 hom kev hloov kho, peb muaj txhua yam uas koj tuaj yeem xav txog.
Cov neeg siv khoom feem ntau tuaj rau peb nrog cov ntaub ntawv thiab nug: "Koj puas tuaj yeem ua qhov kev hloov kho no?" Peb ua tib zoo saib, thiab feem ntau cov lus teb yog: "Peb tau ua tiav, thiab peb tuaj yeem ua tau zoo dua." Vim tias dhau 20 xyoo dhau los, peb tau sau ntau qhov kev paub dhau los thiab kev ua tsis tiav nrog kev txiav kom paub tias dab tsi ua haujlwm thiab qhov tsis ua haujlwm.
2. Kev hloov kho kev sib txuas ua ke? Peb tau disassembled ntau ntawm lawv.
Qee cov peptides yuav tsum nqa ntau qhov kev hloov kho tib lub sijhawm: N- daim ntawv lo fluorescent, nruab nrab phosphorylation, C-terminal amidation. Qhov kev sib xyaw ua ke no yog qhov xav tau ntawm cov tswv yim synthesis, thiab ib kauj ruam tsis raug yuav ua rau tag nrho tsis ua tiav. Peb optimize cov amino acid tiv thaiv lub tswv yim thiab coupling tej yam kev mob los xyuas kom meej tias txhua qhov kev hloov kho nyob rau hauv qhov chaw thiab tsis cuam tshuam rau ib leeg.
Ib zaj dab neeg tseeb: ib tus neeg siv khoom xav tsim cov 25 peptide nrog 3 kev hloov kho (phosphorylation + biotin + fluorescent labeling). Tom qab nug ntau lub tuam txhab, lawv hais tias lawv ua tsis tau lossis hais tias nws yuav siv sijhawm tsawg kawg 6 lub hlis. Tom qab kev soj ntsuam, peb rov tsim kho cov txheej txheem synthesis thiab xa cov khoom hauv 6 lub lis piam, nrog kev ua kom huv ntawm ntau dua 92%, thiab cov neeg siv khoom txawm hais rau peb hauv kev lees paub thaum nws xa tsab xov xwm tom qab.
3. Kev hloov kho nyuaj uas peb ua tau zoo dua
Ntau daim ntawv cog lus disulfide: mus txog 4 khub kom paub meej qhov tseeb.
Stapled peptide: tag nrho cov hydrocarbon saw stapled, txhim kho helical stability thiab cell nkag.
Glycosylation: O-conjugation, N-conjugation, complex qab zib chains kuj tuaj yeem ua rau.
Hydrophobic ntev peptide: hydrophobic peptide nws tus kheej, nyuaj rau solubilize nrog kev hloov kho? Peb muaj ib tug tshwj xeeb hnyav system uas saib xyuas qhov ntawd.
4. Kev tswj kom zoo tsis yog ib qho qauv, txhua tus peptide muaj "ID card".
Kev tswj xyuas zoo ntawm kev hloov pauv peptides muaj teeb meem ntau dua li cov peptides zoo tib yam - tsis yog peb yuav tsum tau kuaj xyuas seb qhov hnyav molecular puas yog qhov tseeb lossis tsis yog, tab sis peb kuj yuav tsum paub meej tias qhov chaw hloov kho puas yog qhov teev tseg. Peb niaj hnub muab:
MS / MS Secondary Mass spectrometry: cov pov thawj ncaj qha tias qhov kev hloov kho txuas nrog rau txoj haujlwm koj xav tau.
HPLC purity tsom xam: qhov purity yog dab tsi, tsis muaj lus dag.
Kev ntsuam xyuas yeem: peptide cov ntsiab lus, endotoxin, noo noo, amino acid muaj pes tsawg leeg tsom xam, ntxiv raws li xav tau.
5. Los ntawm milligrams mus rau kilograms, los ntawm R & D mus rau ntau lawm
Ib qho kev hloov pauv peptide ua tau zoo nyob rau theem kev tshuaj ntsuam, thiab tom qab ntawd koj xav kom nws loj dua rau kev sim tsiaj lossis txawm mus kuaj mob? Tsis muaj teeb meem. Peb tuaj yeem ua tiav qhov kev sib txuas los ntawm me me mus rau cGMP ntau lawm ntawm tib lub platform, thiab cov kev hloov kho ntawm cov txheej txheem amplification tseem zoo ib yam, uas txuag cov teeb meem ntawm cov txheej txheem hloov mus rau theem tom ntej.
Dab tsi tuaj yeem hloov kho Peptide pab koj ua tiav?
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Kev Tshawb Fawb Cov Lus Qhia |
Pom zoo hom kev hloov kho |
Tus nqi ntawm kev hloov kho |
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Cell Imaging thiab Tracing |
FITC (Fluorescein Isothiocyanate), TAMRA (Tetramethylrhodamine), Cy5, biotin |
Visualization thiab localization, taug qab ntawm intracellular peptide faib |
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Kev Tshawb Fawb Kev Hloov Pauv |
Phosphorylation (phosphorylation), acetylation (acetylation), methylation (methylation) |
Mimicking ncej-txhais lus txhais los kawm txog kev ua haujlwm ntawm kev hloov kho |
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Tshuaj ntsuam xyuas |
Stapled peptide, PEGylation, fatty acid hloov kho |
Txhim kho stability, permeability, thiab kev ua haujlwm |
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Enzymatic Analysis |
Fluorescence quenching khub (FRET peptide: Förster Resonance Energy Transfer peptide), phosphorylated peptide |
Tiag -lub sij hawm saib xyuas kev ua enzyme, tshuaj xyuas cov inhibitors |
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Kev tsim tshuaj tiv thaiv |
Glycosylation (glycosylation), lipidation (lipid hloov kho) |
Txhim kho immunogenicity, ua raws li cov neeg hauv zos antigens |
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Qauv Biology |
Isotopic labeling (¹⁵N: nitrogen-15, ¹³C: carbon-13), selenomethionine (a selenium-substituted methionine) |
NMR (Nuclear Magnetic Resonance) thiab X-ray crystallography kev tshawb fawb |
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Diagnostic Reagents |
Biotin, fluorescent labeling, DOTA (1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid) |
Nrhav kev sojntsuam, kuaj xyuas kev sojntsuam, kev sojntsuam duab |
Kev xa khoom thiab kev tswj xyuas zoo
Khoom siv:lyophilized hmoov, centrifuge raj los yog syringes.
Cov ntaub ntawv:COA daim ntawv qhia (nrog HPLC profile thiab MS profile), synthesis report (optional).
Kev sim ntxiv:peptide cov ntsiab lus, noo noo, endotoxin, microbiology, amino acid muaj pes tsawg leeg, ib theem validation - ntawm kev thov.
Peb nkag siab tias qhov zoo ntawm kev hloov pauv peptide ncaj qha txiav txim siab qhov ua tiav lossis tsis ua tiav ntawm koj qhov kev sim tom ntej. Yog li ntawd, ua ntej txhua peptide raug xa tawm, peb yuav xyuas nws ntau zaus kom paub meej tias qhov chaw hloov kho yog qhov tseeb, qhov purity yog raws li tus qauv, thiab cov ntaub ntawv tuaj yeem taug qab.
Peb qhov piv txwv tiag tiag, saib seb lawv siv li cas
Case 1: Xav tau los ntawm lub chaw kho mob cancer saum toj kawg nkaus
Lawv xav tau p53-derived peptide nrog kev hloov kho dual (cov ntawv cim fluorescent + phosphorylation) los kawm txog kev sib cuam tshuam ntawm cov protein. Qhov teeb meem yog tias peptide nws tus kheej yog hydrophobic thiab yuav luag insoluble tom qab ntxiv ob qhov kev hloov kho. Peb nrhiav tau ib txoj hauv kev los qhia ib ntus solubilization tag, thiab tom qab ntawd tshem nws tom qab kev sib txuas, thiab thaum kawg tau txais lub hom phiaj peptide. Tus neeg siv khoom siv nws los ua cov ntaub ntawv tseem ceeb, thiab tsab xov xwm tau luam tawm hauv Cancer Cell.
Case 2: European biotech tuam txhab
Lawv tab tom tsim peptide-coupled tshuaj thiab yuav tsum tau txuas cov tshuaj lom molecules ntawm cov haujlwm tshwj xeeb. Peb muab cov hloov pauv peptide nrog cov pab pawg tiv thaiv orthogonal ua ntej, thiab tus neeg siv khoom tau ua qhov txuas ntxiv los ntawm lawv tus kheej. Vim tias peb tswj hwm qhov chaw hloov kho kom raug, lawv muaj kev xaiv zoo hauv kev sib txuas thiab cov txiaj ntsig tau siab tshaj qhov xav tau.
Case 3: Pab neeg tshawb fawb hauv tsev
They need a batch of stapled peptides for antibacterial activity screening. The stapled peptides were difficult to synthesize and the yield was generally low. We optimized the introduction of olefinic amino acid and ring-closing conditions, and finally delivered a stapled peptide with purity >90% thiab yield 30% siab tshaj qhov lawv xav tau, thiab kev tshuaj ntsuam xyuas tau ua tiav.
Ntau tshaj li qhov hloov pauv peptides, peb tuaj yeem ua ntau yam ntxiv
Tau txais -zoo hloov peptide feem ntau tsuas yog thawj kauj ruam hauv kev tshawb fawb. Tej zaum yuav muaj ntau yam ua raws li:
Txheeb xyuas cov haujlwm
Scale up the synthesis for animal studies
Tsim cov txheej txheem rau kev tshawb fawb soj ntsuam
cGMP ntau lawm rau kev sim tshuaj
Kev tshawb fawb-Peptide tuaj yeem nrog koj txhua txoj hauv kev. Peb tsis yog muag koj peptide xwb, peb yog koj tus khub thev naus laus zis los ntawm kev tshawb pom mus rau kev loj hlob. Thaum koj xaiv peb Kev Hloov Kho Peptide, koj muaj ib pab neeg tom qab koj nkag siab txog chemistry thiab biology.
Tham txog koj cov kev xav tau?
Qhia rau peb koj cov ntaub ntawv ua ntu zus (lossis lub hom phiaj keeb kwm yav dhau), hom kev hloov kho koj xav tau, kom muaj nuj nqis thiab purity yuav tsum. tsis pub dhau 24 teev koj yuav tau txais cov lus qhia txog kev ua tau zoo thiab nqe lus.
