Oxytocin extraction: Siv 100g qhuav * Pterocarya stenoptera * hmoov thiab 30g quartz hmoov, muab tso rau hauv ib lub pob zeb, ntxiv dej distilled, thiab rho tawm plaub zaug siv tib txoj kev. Cov dej distilled ntxiv yog 1.4L ob zaug thiab 1.3L ob zaug. Txhua qhov kev rho tawm siv sijhawm 45 feeb, tom qab ntawd centrifugation. Sau cov kua, thiab rho tawm cov residue dua. Muab cov plaub tshem tawm kom tau txais cov tshuaj oxytocin. Ntawd yog: qhuav * Pterocarya stenoptera * hmoov [dej, pob zeb] → oxytocin extract (chromatographic sib cais).
Ntxiv 1500g ntawm pre- kho hluavtaws zeolite rau 20L ntawm 0.25% acetic acid tov, do, thiab ncuav rau hauv ib qho kev sib pauv kem. Thaum cov tshuaj acetic acid poob mus rau 2-3cm saum toj no zeolite nto, ntxiv cov extract. Sau cov kua dawb turbid (feem ntau muaj oxytocin, vim nws pI 7.7, thaum vasopressin muaj pI 10.9, ua rau nws yooj yim adsorbed raws li ib tug zoo ion) ntawm tus nqi txaus txaus. Thaum cov qib extract poob mus rau zeolite nto, tam sim ntawd ntxiv dej distilled thiab txuas ntxiv mus sau cov turbid kua kom txog thaum tag nrho cov kua tau ntws tawm. Cov tshuaj turbid tau hloov mus rau pH 3.5 nrog glacial acetic acid, sai rhuab mus rau 95℃nyob rau hauv ib tug da dej thiab tuav rau 3 feeb, ces sai txias thiab refrigerated ib hmos. Cov txheej txheem yog: oxytocin extract [HAc, hluavtaws zeolite kem] → turbid tov [HAc, rhuab] → cais tov (adsorption, elution). Hnub tom qab, cov kua hauv tub yees tau lim, thiab 10% bentonite slurry (3 mL ib 100 mL) tau ntxiv rau cov lim dej nrog nplawm. Adsorption tau ua tiav rau 1 teev, tom qab ntawd centrifugation. Lub supernatant raug soj ntsuam nrog sulfosalicylic acid test tov; Tsis muaj nag lossis daus qhia tias ua tiav adsorption. Yog tias tsis tiav adsorption tshwm sim, qhov adsorption tau rov ua dua, thiab ob lub bentonite slurry nag los ua ke. Cov bentonite tau eluted plaub zaug nrog 1% acetic acid (1.6 L, 1.4 L, 1.2 L, thiab 0.8 L feem). Txhua eluent tau rhuab mus rau 80 degree, chlorobutanol tau ntxiv, thiab qhov kub thiab txias tau ntxiv mus rau 95 degree, ces txias sai heev rau 25 degree. Cov eluents tau lim los yog centrifuged, thiab plaub filtrates tau ua ke los txiav txim siab lub zog thiab vasopressin txwv. Ntawd yog, kev npaj cov kua hauv tub yees [filtration] → filtrate [soap clay slurry] → adsorbent [1% HAc] → eluent oxytocin txhaj.
