Thaum siv peptides, peb feem ntau ntsib cov ntsuas peptide purity thiab peptide cov ntsiab lus. Ntau tus neeg yuav xav tias vim li cas lawv cov peptide purity yog 95% tab sis cov ntsiab lus peptide tsuas yog 80%. Qhov no yog vim cov no yog ob lub tswv yim sib txawv kiag li.
Peptide Purity: Peptide purity yog hais txog tus nqi ntawm lub hom phiaj peptide kuaj pom ntawm 214 nm (214 nm yog qhov nqus nqus ntawm cov peptide saw) siv HPLC kev tshuaj xyuas.
Cov ntsiab lus Peptide: Qhov no yog hais txog feem pua ntawm peptides txheeb ze rau cov tsis yog -peptide cov tshuaj hauv cov qauv.
Lub purity ntawm peptide yog hais txog cov ntsiab lus ntawm lub hom phiaj peptide kuaj pom los ntawm HPLC ntawm 214 nm (214 nm yog qhov nqus nqus ntawm peptide saw). Dhau li ntawm lub hom phiaj peptide, HPLC tuaj yeem tshawb xyuas cov impurities xws li: cov kab lus tshem tawm (cov phiaj xwm kab ke uas ploj lawm ib lossis ntau cov amino acid residues), truncated sequences (sequences generated thaum tiv thaiv), thiab incompletely deprotected sequences (ua rau cov txheej txheem synthesis los yog cov txheej txheem cleavage zaum kawg). Txawm li cas los xij, HPLC tsis tuaj yeem ntes dej thiab cov ntsev seem xws li trifluoroacetate (TFA) thiab acetate. Thaum lub sij hawm peptide synthesis, cov kab mob peptide yog cleaved los ntawm cov resin siv TFA, thiab ib tug me me ntawm TFA kuj generated thaum lub sij hawm purification. Yog li ntawd, cov dawb amino terminus thiab lwm sab chains ntawm peptide, xws li Arg, Lys, thiab Nws, tuaj yeem tsim me me ntawm TFA impurities. Lwm qhov teeb meem yog dej noo. Peptides feem ntau yog lub cev qhuav dej siv lyophilization, tab sis txawm li ntawd los, dej yuav muaj nyob rau ntau qib nyob ntawm seb covalent binding muaj peev xwm. Yog li, xa peptides feem ntau muaj TFA ntsev thiab cov kab dej ntau, uas tsis tuaj yeem kuaj pom los ntawm HPLC, yog li ua rau muaj qhov sib txawv ntawm peptide cov ntsiab lus thiab purity.
